Cas9 Nuclease Protein
GEEN
10 μM
Catalog No. ABIN3188187
Quick Overview for Cas9 Nuclease Protein (ABIN3188187)
Application
Genome Editing with Engineered Nucleases (GEEN)
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Characteristics
- The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided, endonuclease tool in genome editing which allows for very specific genomic disruption and replacement. Guided by a target-specific, single guide RNA (sgRNA), the Cas9 Nuclease Protein serves to unwind the genomic DNA duplex and cleave both strands upon recognition of the target sequence by the sgRNA. The resulting double-stranded break gets repaired by the non-homologous end joining (NHEJ) pathway, leading to a disruption in the open reading frame of the targeted gene.
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Components
- Enzyme supplied with 10X Reaction Buffer
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Restrictions
- For Research Use only
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Concentration
- 10 μM
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Buffer
- 10 mM Tris-HCl ( pH 7.4), 0.1 mM EDTA, 1 mM DTT, 300 mM NaCl, and 50 % (v/v) Glycerol.
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Storage
- -20 °C
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