2xTransTaq®-T PCR SuperMix ( +dye)
PCR
Quick Overview for 2xTransTaq®-T PCR SuperMix ( +dye) (ABIN5519418)
Application
Polymerase Chain Reaction (PCR)
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Purpose
- suitable for high fidelity TA cloning
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Brand
- TransTaq®
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Specificity
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TransTaq®-T PCR SuperMix offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.
Extension rate is about 1-2 kb/min.
Template-independent "A" can be generated at the 3' end of the PCR product. PCR products can be cloned into pEASY®-T vectors.
Amplification of genomic DNA fragment up to 8 kb. -
Characteristics
- TransTaq®-T PCR SuperMix is a ready-to-use mixture of TransTaq®-T DNA Polymerase, dNTPs and optimized buffer. The SuperMix is provided at 2x concentration and used at 1x concentration by adding template, primers and H2O. Efficiency of PCR products with "A" is equal to EasyTaq® DNA polymerase. It is more suitable for high fidelity TA cloning.
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Components
- 2xTransTaq®-T PCR SuperMix (+dye), ddH2O
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Application Notes
- Complex templates, TA Cloning
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Restrictions
- For Research Use only
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Storage
- -20 °C
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Storage Comment
- at -20°C for two years
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Expiry Date
- 24 months
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