Taq DNA Polymerase
- Thermus aquaticus
- Polymerase Chain Reaction (PCR)
- Taq DNA Polymerase is a thermostable DNA Polymerase isolated from an E. coli strain that carries the Taq DNA polymerase gene. Taq DNA Polymerase is the most common polymerase used for PCR.
- Application Notes
- The applications of Taq DNA Polymerase include the following: PCR 3' A-tailing of blunt ends Primer extension DNA sequencing.
Terminal transferase activity: Taq DNA Polymerase has terminal transferase activity, which results in the addition of a single nucleotide (adenosine) at the 3' end of the extension product.
High purity: No contamination activity has been detected in standard test reactions.
Terminal Transferase Activity: A single nucleotide (adenosine) is added to the 3' end of the extension product.
High-purity: No contamination activity has been detected in standard test reactions.
Unit Definition: one unit is defined as the amount of enzyme that can incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C.
- For Research Use only
- 500 mM KCl, 100 mM Tris HCl (pH 9.0 at 25°C), 15 mM MgCl2, 1% Triton X-100 Buffer. This buffer is optimized for use with 200 µM dNTPs. Note: If the reaction is performed without this buffer, then add 0.1% Triton X-100 (final concentration) to ensure high activity. Concentration: Taq is delivered in 5 units/µl in 20 mM Tris HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 0.1% Triton X-100 and 50% glycerol.
- -20 °C
- Storage Comment
- Store the product at -20°C. The enzyme can be shipped at room temperature or even 37°C for seven days without any loss of activity.
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