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Taq DNA Polymerase Enzyme

Pubmed (103 references)
Catalog No. ABIN1536547
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  • Species
    Thermus aquaticus
    Polymerase Chain Reaction (PCR)
    Taq DNA Polymerase is a thermostable DNA Polymerase isolated from an E. coli strain that carries the Taq DNA polymerase gene. Taq DNA Polymerase is the most common polymerase used for PCR.
  • Application Notes
    The applications of Taq DNA Polymerase include the following: PCR 3' A-tailing of blunt ends Primer extension DNA sequencing.

    Terminal transferase activity: Taq DNA Polymerase has terminal transferase activity, which results in the addition of a single nucleotide (adenosine) at the 3' end of the extension product.
    High purity: No contamination activity has been detected in standard test reactions.
    Terminal Transferase Activity: A single nucleotide (adenosine) is added to the 3' end of the extension product.
    High-purity: No contamination activity has been detected in standard test reactions.
    Unit Definition: one unit is defined as the amount of enzyme that can incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C.

    For Research Use only
  • Format
    500 mM KCl, 100 mM Tris HCl (pH 9.0 at 25°C), 15 mM MgCl2, 1% Triton X-100 Buffer. This buffer is optimized for use with 200 µM dNTPs. Note: If the reaction is performed without this buffer, then add 0.1% Triton X-100 (final concentration) to ensure high activity. Concentration: Taq is delivered in 5 units/µl in 20 mM Tris HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 0.1% Triton X-100 and 50% glycerol.
    -20 °C
    Storage Comment
    Store the product at -20°C. The enzyme can be shipped at room temperature or even 37°C for seven days without any loss of activity.
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    González, Brito, González: "Identification of glycoproteins secreted by wild-type Botrytis cinerea and by protein O-mannosyltransferase mutants." in: BMC microbiology, Vol. 14, pp. 254, (2015) (PubMed).

    Boyd, Tennant, Melendez, Toema, Galen, Geddes, Levine: "Adaptation of red blood cell lysis represents a fundamental breakthrough that improves the sensitivity of Salmonella detection in blood." in: Journal of applied microbiology, Vol. 118, Issue 5, pp. 1199-209, (2015) (PubMed).

    Miersch, Li, Hanna, McLaughlin, Hornsby, Matsuguchi, Paduch, Sääf, Wells, Koide, Kossiakoff, Sidhu: "Scalable high throughput selection from phage-displayed synthetic antibody libraries." in: Journal of visualized experiments : JoVE, Issue 95, pp. 51492, (2015) (PubMed).

    Thekke-Veetil, Polashock, Marn, Plesko, Schilder, Keller, Martin, Tzanetakis: "Population structure of blueberry mosaic associated virus: Evidence of reassortment in geographically distinct isolates." in: Virus research, Vol. 201, pp. 79-84, (2015) (PubMed).

    Maxfield-Taylor, Mujic, Rao: "First detection of the larval chalkbrood disease pathogen Ascosphaera apis (Ascomycota: Eurotiomycetes: Ascosphaerales) in adult bumble bees." in: PLoS ONE, Vol. 10, Issue 4, pp. e0124868, (2015) (PubMed).

    Frías, Brito, González, González: "The phytotoxic activity of the cerato-platanin BcSpl1 resides in a two-peptide motif on the protein surface." in: Molecular plant pathology, Vol. 15, Issue 4, pp. 342-51, (2014) (PubMed).

    Acanda, Martínez, Prado, González, Rey: "EMS mutagenesis and qPCR-HRM prescreening for point mutations in an embryogenic cell suspension of grapevine." in: Plant cell reports, Vol. 33, Issue 3, pp. 471-81, (2014) (PubMed).

    Zhang, Liu, Liu, Zhang, Cao, Ye, Wang, Zhang, Xiao, Yang, Li, Bai: "Transcriptome profiling of a multiple recurrent muscle-invasive urothelial carcinoma of the bladder by deep sequencing." in: PLoS ONE, Vol. 9, Issue 3, pp. e91466, (2014) (PubMed).

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    Gandhirajan, Meng, Chandramoorthy, Mallilankaraman, Mancarella, Gao, Razmpour, Yang, Houser, Chen, Koch, Wang, Soboloff, Gill, Madesh: "Blockade of NOX2 and STIM1 signaling limits lipopolysaccharide-induced vascular inflammation." in: The Journal of clinical investigation, Vol. 123, Issue 2, pp. 887-902, (2013) (PubMed).

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    Chiorazzi, Rui, Yang, Ceribelli, Tishbi, Maurer, Ranuncolo, Zhao, Xu, Chan, Jaffe, Gascoyne, Campo, Rosenwald, Ott, Delabie, Rimsza, Shaham, Staudt: "Related F-box proteins control cell death in Caenorhabditis elegans and human lymphoma." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 10, pp. 3943-8, (2013) (PubMed).

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    Jun, Rouf Mian, Michel: "Genetic mapping of three quantitative trait loci for soybean aphid resistance in PI 567324." in: Heredity, Vol. 111, Issue 1, pp. 16-22, (2013) (PubMed).

    Doyle, Oudemans, Rehner, Litt: "Habitat and host indicate lineage identity in Colletotrichum gloeosporioides s.l. from wild and agricultural landscapes in North America." in: PLoS ONE, Vol. 8, Issue 5, pp. e62394, (2013) (PubMed).

    Phillips, Dekoning, Brunelli, Faber-Hammond, Hansen, Christensen, Renn, Thorgaard: "Characterization of the OmyY1 Region on the Rainbow Trout Y Chromosome." in: International journal of genomics, Vol. 2013, pp. 261730, (2013) (PubMed).

    Wang, Sager, Cui, Zhang, Lu, Lee: "Salicylic acid regulates Plasmodesmata closure during innate immune responses in Arabidopsis." in: The Plant cell, Vol. 25, Issue 6, pp. 2315-29, (2013) (PubMed).

    Jun, Rouf Mian, Michel: "Genetic mapping revealed two loci for soybean aphid resistance in PI 567301B." in: TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, Vol. 124, Issue 1, pp. 13-22, (2012) (PubMed).

    Reese, Moore, McLachlan: "Limited effects of bile acids and small heterodimer partner on hepatitis B virus biosynthesis in vivo." in: Journal of virology, Vol. 86, Issue 5, pp. 2760-8, (2012) (PubMed).

    Verhoeven, Altstadt, Savinov: "Intracellular detection and evolution of site-specific proteases using a genetic selection system." in: Applied biochemistry and biotechnology, Vol. 166, Issue 5, pp. 1340-54, (2012) (PubMed).

    Mulvey, Sacksteder, Einck, Nacy: "Generation of a novel nucleic acid-based reporter system to detect phenotypic susceptibility to antibiotics in Mycobacterium tuberculosis." in: mBio, Vol. 3, Issue 2, (2012) (PubMed).

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    Adikaram, Perera, Wijesundera: "Geographical profile of rpoB gene mutations in rifampicin resistant Mycobacterium tuberculosis isolates in Sri Lanka." in: Microbial drug resistance (Larchmont, N.Y.), Vol. 18, Issue 5, pp. 525-30, (2012) (PubMed).

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    Lema, Slane, Salvesen, Godwin: "Variation in gene transcript profiles of two V1a-type arginine vasotocin receptors among sexual phases of bluehead wrasse (Thalassoma bifasciatum)." in: General and comparative endocrinology, Vol. 179, Issue 3, pp. 451-64, (2012) (PubMed).

    Zhou, Zhang, Wang, Gao, Xu: "Identification of a novel gene fusion RNF213?SLC26A11 in chronic myeloid leukemia by RNA-Seq." in: Molecular medicine reports, Vol. 7, Issue 2, pp. 591-7, (2012) (PubMed).

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    Hao, Yan, Zhou, Liu, Cai, Ouyang: "The effect of AmtR on growth and amino acids production in Corynebacterium glutamicum." in: Prikladnaia biokhimiia i mikrobiologiia, Vol. 46, Issue 6, pp. 611-6, (2011) (PubMed).

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    Muturi, Ouma, Malele, Ngure, Rutto, Mithöfer, Enyaru, Masiga: "Tracking the feeding patterns of tsetse flies (Glossina genus) by analysis of bloodmeals using mitochondrial cytochromes genes." in: PLoS ONE, Vol. 6, Issue 2, pp. e17284, (2011) (PubMed).

    Omondi, van den Berg, Masiga, Schulthess: "Phylogeographic structure of Teretrius nigrescens (Coleoptera: Histeridae) predator of the invasive post harvest pest Prostephanus truncatus (Coleoptera: Bostrichidae)." in: Bulletin of entomological research, Vol. 101, Issue 5, pp. 521-32, (2011) (PubMed).

    Malu, Bélanger, Desautels, Kelendji, Dalko, Sanchez-Dardon, Leng, Bucala, Satoskar, Scorza: "Macrophage migration inhibitory factor: a downregulator of early T cell-dependent IFN-gamma responses in Plasmodium chabaudi adami (556 KA)-infected mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 186, Issue 11, pp. 6271-9, (2011) (PubMed).

    Jun, Michel, Mian: "Development of soybean aphid genomic SSR markers using next generation sequencing." in: Genome / National Research Council Canada = Génome / Conseil national de recherches Canada, Vol. 54, Issue 5, pp. 360-7, (2011) (PubMed).

    Renshaw, Douglas, Rexroad Iii, Jobity, Gold: "Isolation and characterization of microsatellite markers in the Serra Spanish mackerel, Scomberomorus brasiliensis." in: Molecular ecology resources, Vol. 9, Issue 3, pp. 835-8, (2011) (PubMed).

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    Forbes, Powell, Lobo, Noor, Feder: "PERMANENT GENETIC RESOURCES: Polymorphic microsatellite loci for Diachasma alloeum (Hymenoptera: Braconidae)." in: Molecular ecology resources, Vol. 8, Issue 2, pp. 373-6, (2011) (PubMed).

    Zhou, Kantartzi, Wen, Newman, Hajimorad, Rupe, Tzanetakis: "Molecular characterization of a new Tospovirus infecting soybean." in: Virus genes, Vol. 43, Issue 2, pp. 289-95, (2011) (PubMed).

    Frías, González, Brito: "BcSpl1, a cerato-platanin family protein, contributes to Botrytis cinerea virulence and elicits the hypersensitive response in the host." in: The New phytologist, Vol. 192, Issue 2, pp. 483-95, (2011) (PubMed).

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    Lee, Wang, Cui, Sager, Modla, Czymmek, Zybaliov, van Wijk, Zhang, Lu, Lakshmanan: "A plasmodesmata-localized protein mediates crosstalk between cell-to-cell communication and innate immunity in Arabidopsis." in: The Plant cell, Vol. 23, Issue 9, pp. 3353-73, (2011) (PubMed).

    Choi, Kim, Ito, Lee, Li, Monahan, Wen, Wilson, Kurima, Saunders, Petralia, Wangemann, Friedman, Griffith: "Mouse model of enlarged vestibular aqueducts defines temporal requirement of Slc26a4 expression for hearing acquisition." in: The Journal of clinical investigation, Vol. 121, Issue 11, pp. 4516-25, (2011) (PubMed).

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    Cappadocia, Maréchal, Parent, Lepage, Sygusch, Brisson: "Crystal structures of DNA-Whirly complexes and their role in Arabidopsis organelle genome repair." in: The Plant cell, Vol. 22, Issue 6, pp. 1849-67, (2010) (PubMed).

    Vuong, Sleper, Shannon, Nguyen: "Novel quantitative trait loci for broad-based resistance to soybean cyst nematode (Heterodera glycines Ichinohe) in soybean PI 567516C." in: TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, Vol. 121, Issue 7, pp. 1253-66, (2010) (PubMed).

    Bai, Zhang, Orantes, Jun, Mittapalli, Mian, Michel: "Combining next-generation sequencing strategies for rapid molecular resource development from an invasive aphid species, Aphis glycines." in: PLoS ONE, Vol. 5, Issue 6, pp. e11370, (2010) (PubMed).

    Ubayasena, Bett, Taran, Vijayan, Warkentin: "Genetic control and QTL analysis of cotyledon bleaching resistance in green field pea (Pisum sativum L.)." in: Genome / National Research Council Canada = Génome / Conseil national de recherches Canada, Vol. 53, Issue 5, pp. 346-59, (2010) (PubMed).

    Datta, Bucks, Koley, Lim, Savinov: "Functional profiling of p53-binding sites in Hdm2 and Hdmx using a genetic selection system." in: Bioorganic & medicinal chemistry, Vol. 18, Issue 16, pp. 6099-108, (2010) (PubMed).

    Frade, Reyes-Nivia, Faria, Kaandorp, Luttikhuizen, Bak: "Semi-permeable species boundaries in the coral genus Madracis: introgression in a brooding coral system." in: Molecular phylogenetics and evolution, Vol. 57, Issue 3, pp. 1072-90, (2010) (PubMed).

    Roux-Michollet, Schimel, Holden: "Pushing the limits for amplifying BrdU-labeled DNA encoding 16S rRNA: DNA polymerase as the determining factor." in: Journal of microbiological methods, Vol. 83, Issue 3, pp. 312-6, (2010) (PubMed).

    Martin, Zhou, Tzanetakis: "Blueberry latent virus: an amalgam of the Partitiviridae and Totiviridae." in: Virus research, Vol. 155, Issue 1, pp. 175-80, (2010) (PubMed).

    Curtis, Belcram, Bollmann, Tominey, Hoffman, Mercier, Hays et al.: "Reciprocal chromosome translocation associated with TDNA-insertion mutation in Arabidopsis: genetic and cytological analyses of consequences for gametophyte development and for construction of doubly ..." in: Planta, Vol. 229, Issue 4, pp. 731-45, (2009) (PubMed).

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    Dan, Yang, Zheng: "A negative regulatory role for auxin in sulphate deficiency response in Arabidopsis thaliana." in: Plant molecular biology, Vol. 63, Issue 2, pp. 221-35, (2007) (PubMed).

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