- Thermus aquaticus
- Polymerase Chain Reaction (PCR)
- Taq DNA Polymerase is a thermostable DNA Polymerase isolated from an E. coli strain that carries the Taq DNA polymerase gene. Taq DNA Polymerase is the most common polymerase used for PCR.
- Application Notes
- The applications of Taq DNA Polymerase include the following: PCR 3' A-tailing of blunt ends Primer extension DNA sequencing.
Terminal transferase activity: Taq DNA Polymerase has terminal transferase activity, which results in the addition of a single nucleotide (adenosine) at the 3' end of the extension product.
High purity: No contamination activity has been detected in standard test reactions.
Terminal Transferase Activity: A single nucleotide (adenosine) is added to the 3' end of the extension product.
High-purity: No contamination activity has been detected in standard test reactions.
Unit Definition: one unit is defined as the amount of enzyme that can incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C.
- For Research Use only
- 500 mM KCl, 100 mM Tris HCl (pH 9.0 at 25°C), 15 mM MgCl2, 1% Triton X-100 Buffer. This buffer is optimized for use with 200 µM dNTPs. Note: If the reaction is performed without this buffer, then add 0.1% Triton X-100 (final concentration) to ensure high activity. Concentration: Taq is delivered in 5 units/µl in 20 mM Tris HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 0.1% Triton X-100 and 50% glycerol.
- -20 °C
- Storage Comment
- Store the product at -20°C. The enzyme can be shipped at room temperature or even 37°C for seven days without any loss of activity.
Lee, Rollwagen-Bollens, Bollens, Faber-Hammond: "Environmental influence on cyanobacteria abundance and microcystin toxin production in a shallow temperate lake." in: Ecotoxicology and environmental safety, Vol. 114, pp. 318-25, 2015 (PubMed).
González, Brito, González: "Identification of glycoproteins secreted by wild-type Botrytis cinerea and by protein O-mannosyltransferase mutants." in: BMC microbiology, Vol. 14, pp. 254, 2015 (PubMed).
Boyd, Tennant, Melendez, Toema, Galen, Geddes, Levine: "Adaptation of red blood cell lysis represents a fundamental breakthrough that improves the sensitivity of Salmonella detection in blood." in: Journal of applied microbiology, Vol. 118, Issue 5, pp. 1199-209, 2015 (PubMed).
Miersch, Li, Hanna, McLaughlin, Hornsby, Matsuguchi, Paduch, Sääf, Wells, Koide, Kossiakoff, Sidhu: "Scalable high throughput selection from phage-displayed synthetic antibody libraries." in: Journal of visualized experiments : JoVE, Issue 95, pp. 51492, 2015 (PubMed).
Thekke-Veetil, Polashock, Marn, Plesko, Schilder, Keller, Martin, Tzanetakis: "Population structure of blueberry mosaic associated virus: Evidence of reassortment in geographically distinct isolates." in: Virus research, Vol. 201, pp. 79-84, 2015 (PubMed).
Maxfield-Taylor, Mujic, Rao: "First detection of the larval chalkbrood disease pathogen Ascosphaera apis (Ascomycota: Eurotiomycetes: Ascosphaerales) in adult bumble bees." in: PLoS ONE, Vol. 10, Issue 4, pp. e0124868, 2015 (PubMed).
Frías, Brito, González, González: "The phytotoxic activity of the cerato-platanin BcSpl1 resides in a two-peptide motif on the protein surface." in: Molecular plant pathology, Vol. 15, Issue 4, pp. 342-51, 2014 (PubMed).
Acanda, Martínez, Prado, González, Rey: "EMS mutagenesis and qPCR-HRM prescreening for point mutations in an embryogenic cell suspension of grapevine." in: Plant cell reports, Vol. 33, Issue 3, pp. 471-81, 2014 (PubMed).
Zhang, Liu, Liu, Zhang, Cao, Ye, Wang, Zhang, Xiao, Yang, Li, Bai: "Transcriptome profiling of a multiple recurrent muscle-invasive urothelial carcinoma of the bladder by deep sequencing." in: PLoS ONE, Vol. 9, Issue 3, pp. e91466, 2014 (PubMed).
Boyd, Tennant, Saague, Simon, Muhsen, Ramachandran, Cross, Galen, Pasetti, Levine: "Serum bactericidal assays to evaluate typhoidal and nontyphoidal Salmonella vaccines." in: Clinical and vaccine immunology : CVI, Vol. 21, Issue 5, pp. 712-21, 2014 (PubMed).
Zhan, Bailey, Heath, Macisaac: "Performance comparison of genetic markers for high-throughput sequencing-based biodiversity assessment in complex communities." in: Molecular ecology resources, Vol. 14, Issue 5, pp. 1049-59, 2014 (PubMed).
Yoon, Hossain, Held, Hou, Kehl, Tromas, Sato, Tabata, Andersen, Stougaard, Ross, Szczyglowski: "Lotus japonicus SUNERGOS1 encodes a predicted subunit A of a DNA topoisomerase VI that is required for nodule differentiation and accommodation of rhizobial infection." in: The Plant journal : for cell and molecular biology, Vol. 78, Issue 5, pp. 811-21, 2014 (PubMed).
Thompson, Taran: "Genetic characterization of the acetohydroxyacid synthase (AHAS) gene responsible for resistance to imidazolinone in chickpea (Cicer arietinum L.)." in: TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, Vol. 127, Issue 7, pp. 1583-91, 2014 (PubMed).
Chuang, Liao, Chang, Wang, Lin, Hsieh: "Ciliated muconodular papillary tumor of the lung: a newly defined peripheral pulmonary tumor with conspicuous mucin pool mimicking colloid adenocarcinoma: a case report and review of literature." in: Pathology international, Vol. 64, Issue 7, pp. 352-7, 2014 (PubMed).
Espino, González, González, Brito: "Efficiency of different strategies for gene silencing in Botrytis cinerea." in: Applied microbiology and biotechnology, Vol. 98, Issue 22, pp. 9413-24, 2014 (PubMed).
Koide, Wellman: "SAH-induced MMP activation and K V current suppression is mediated via both ROS-dependent and ROS-independent mechanisms." in: Acta neurochirurgica. Supplement, Vol. 120, pp. 89-94, 2014 (PubMed).
He, Wang: "DNA consensus sequence motif for binding response regulator PhoP, a virulence regulator of Mycobacterium tuberculosis." in: Biochemistry, Vol. 53, Issue 51, pp. 8008-20, 2014 (PubMed).
Firlej, Doyon, Harwood, Brodeur: "A multi-approach study to delineate interactions between carabid beetles and soybean aphids." in: Environmental entomology, Vol. 42, Issue 1, pp. 89-96, 2013 (PubMed).
Gandhirajan, Meng, Chandramoorthy, Mallilankaraman, Mancarella, Gao, Razmpour, Yang, Houser, Chen, Koch, Wang, Soboloff, Gill, Madesh: "Blockade of NOX2 and STIM1 signaling limits lipopolysaccharide-induced vascular inflammation." in: The Journal of clinical investigation, Vol. 123, Issue 2, pp. 887-902, 2013 (PubMed).
Wang, Kohalmi, Svircev, Wang, Sanfaçon, Tian: "Silencing of the host factor eIF(iso)4E gene confers plum pox virus resistance in plum." in: PLoS ONE, Vol. 8, Issue 1, pp. e50627, 2013 (PubMed).
- Lee, Rollwagen-Bollens, Bollens, Faber-Hammond: "Environmental influence on cyanobacteria abundance and microcystin toxin production in a shallow temperate lake." in: Ecotoxicology and environmental safety, Vol. 114, pp. 318-25, 2015 (PubMed).