EasyPure® Bacteria Genomic DNA Kit uses lysozyme and moderate lysis buffer to lyse cells.
Proteinase K is used for protein digestion and RNase A used for RNA digestion. DNA is specifically bound to silica-based column in hypersaline condition, and DNA is eluted by low salt and high pH solution. This kit is suitable for isolating high quality genomic DNA from Gram-positive and Gram-negative bacteria. The isolated DNA is suitable for PCR, restriction enzyme digestion, and Southern blot. - Fast: the whole process can be completed in 50 minutes. - High yield: DNA yield up to 20 μg.