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Inorganic Pyrophosphatase, Thermostable

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Characteristics Inorganic pyrophosphatase, Thermostable catalyzes the hydrolysis reaction of inorganic pyrophosphate to orthophosphate. P2O7-4 + H2O -> 2HPO4-2.
Source: The source of this protein is a recombinant E. coli strain encoding pyrophosphatase from Thermococcus litoralis.
Unit Definition One unit is defined as the amount of enzyme needed to generate 1 μmol of phosphate in 1 minute from inorganic pyrophosphate under standard reaction conditions (a 10 minute reaction at 75°C in 50 mM Tricine [pH 8.5] , 1 mM MgCl2, 0.32 mM PPi, reaction volume of 0.5 ml).
Restrictions For Research Use only
Concentration 2 U/μL
Buffer 20 mM Tris-HCl, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50 % Glycerol, pH 8.0 at 25 °C.
Storage -20 °C