Phi29 DNA Polymerase
DNA Amp
10 U/μL
Quick Overview for Phi29 DNA Polymerase (ABIN3188235)
Application
DNA Amplification (DNA Amp)
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Characteristics
- Phi29 DNA Polymerase is a highly processive polymerase which exhibits a strong strand-displacement function. These functions allow for highly efficient isothermal amplification of circular or linear DNA templates via rolling circle amplification (RCA), multiple displacement amplification (MDA) and/or whole genome amplification (WGA). Phi29 DNA Polymerase has extremely high fidelity due to its inherent 3'→5' exonuclease activity and can amplify from very small amounts of starting templates.
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Components
- Enzyme supplied with 5X Reaction Buffer
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Unit Definition
- One unit is defined as the amount of Phi29 DNA Polymerase that is required to incorporate 0.5 pmol of dNTP into acid insoluble material in 10 minutes at 30°C.
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Comment
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- Rolling circle amplification (RCA)
- Multiple displacement amplification (MDA)
- Whole genome amplification (WGA)
- DNA template preparation for sequencing
- Proteinprimed DNA amplification
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Restrictions
- For Research Use only
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Concentration
- 10 U/μL
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Buffer
- 50 mM Tris-HCl ( pH 7.5), 0.1 mM EDTA, 1 mM DTT, 100 mM NaCl, 0.5 % Tween-20, 0.5 % NP-40 and 50 % (v/v) Glycerol.
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Storage
- -20 °C
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