Cas9 Nuclease NLS Protein
GEEN
1000 nM
Quick Overview for Cas9 Nuclease NLS Protein (ABIN3188184)
Application
Genome Editing with Engineered Nucleases (GEEN)
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Characteristics
- The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided, endonuclease tool in genome editing which allows for very specific genomic disruption and replacement. Guided by a target-specific, single guide RNA (sgRNA), the Cas9 Nuclease NLS Protein serves to cleave both strands of a DNA duplex upon recognition of the target sequence by the sgRNA. The resulting double-stranded break gets repaired by the non-homologous end joining (NHEJ) pathway, leading to a disruption in the open reading frame of the targeted gene. Cas9 Nuclease NLS contains a SV40 T antigen nuclear localization sequence (NLS) on the C-terminus of the protein.
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Components
- Enzyme supplied with 10X Reaction Buffer
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Restrictions
- For Research Use only
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Concentration
- 1000 nM
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Buffer
- 10 mM Tris-HCl ( pH 7.4), 0.1 mM EDTA, 1 mM DTT, 300 mM NaCl, and 50 % (v/v) Glycerol.
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Storage
- -20 °C
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