EasyScript Plus™ Reverse Transcriptase
cDNA
200 U/μL
Catalog No. ABIN4219166
Quick Overview for EasyScript Plus™ Reverse Transcriptase (ABIN4219166)
Application
cDNA Synthesis (cDNA)
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Purpose
- Synthesize cDNA from complex RNA templates (i.e. secondary structures and high GC content) with EasyScript™ Plus Reverse Transcriptase.
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Brand
- EasyScript Plus™
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Specificity
- EasyScript PlusTM is a novel recombinant RTase that exhibits much higher efficiency in the first-strand cDNA synthesis from RNA templates with secondary structures and high GC content. EasyScript PlusTM is engineered to perform under high temperatures (50 °C - 55 °C) which facilitate the elimination of secondary structures associated with GC-rich RNA templates. Due to this unique feature, EasyScript PlusTM can synthesize full-length cDNA libraries from RNA templates up to 15 kb in length. In addition, EasyScript PlusTM has outstanding proofreading ability, thus it can be utilized for whole genome sequencing.
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Characteristics
- Engineered to perform under high temperatures (45 °C - 55 °C), EasyScript™ Plus Reverse Transcriptase can synthesize full-length cDNA libraries from RNA templates up to 15kb in length. In addition, EasyScript Plus™ Reverse Transcriptase has outstanding proofreading ability due to the presence of a fidelity-enhancing subunit, thus making this RTase an excellent choice for whole genome sequencing.
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Application Notes
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- Synthesizing cDNA from a ssRNA
- DNA primer extension
- Sequencing dsDNA
- Constructing cDNA library
- Constructing libraries for serial analysis for gene expression (SAGE)
- Synthesizing cDNA in rapid amplification of cDNA ends (3' & 5' RACE)
- Producing template for use in RT-PCR or real-time RT-PCR
- Labelling 3'-end of duplex DNA via end-filling reactions
- Generating probes for hybridization -
Comment
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100 x 20 μl reactions
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Restrictions
- For Research Use only
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Concentration
- 200 U/μL
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Buffer
- Enzyme supplied with 5X RT buffer
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Storage
- -20 °C
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Storage Comment
- Store all components at -20°C.
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