FlyCut™ NotI
RD
Quick Overview for FlyCut™ NotI (ABIN5519466)
Application
Restriction Digest (RD)
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Purpose
- Super fast restriction enzyme
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Brand
- FlyCut™
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Specificity
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5'...GC^GGCCGC...3'
3'...CGCCGG^CG...5' -
Characteristics
- FlyCut® NotI is expressed and purified from E.coli that carries the recombinant NotI gene. The molecular weight is 43.3 kDa, with the recognition site at GC^GGCCGC. The reaction is conducted for 5-10 minutes at 37oC, and heat-inactivated at 65oC for 20 minutes. This enzyme is not sensitive to dam or dcm methylation, but sensitive to mammalian CpG methylation.
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Components
- Enzyme, 10X Buffer, 10X DNA Loading Buffer
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Unit Definition
- One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
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Application Notes
- Genomic DNA, plasmid DNA, PCR product
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Comment
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Fast: 5 minutes DNA digestion.
Simple: universal buffer for all restriction enzymes. -
Restrictions
- For Research Use only
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Buffer
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Storage Buffer: 20 mM Tris-HCl pH 7.4, 250 mM NaCl, 0.1 mM EDTA, 1.5 mM DTT, 400 μg/mL BSA, 50 % Glycerol
10xFlyCut® Buffer: 500 mM Tris-Ac pH 7.9, 1 M KAc, 120 mM MgAc2, 1 mg/mL BSA -
Storage
- -20 °C
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Storage Comment
- at -20°C for two years
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Expiry Date
- 24 months
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