TransTaq® DNA Polymerase High Fidelity(HiFi)(with 2.5 mM dNTPs)
PCR
Catalog No. ABIN5519577
Quick Overview for TransTaq® DNA Polymerase High Fidelity(HiFi)(with 2.5 mM dNTPs) (ABIN5519577)
Application
Polymerase Chain Reaction (PCR)
-
-
Purpose
- TransTaq® DNA Polymerase High Fidelity (TransTaq® HiFi DNA Polymerase) contains TransTaq®-T DNA Polymerase and a proofreading 3'-5' exonuclease.
-
Brand
- TransTaq®
-
Specificity
- TransTaq® HiFi DNA Polymerase provides higher specificity and higher amplification efficiency than TransTaq®-T DNA Polymerase. Two different buffers are provided in the kit. TransTaq® HiFi Buffer I is optimized for the amplification of genomic DNA and TransTaq® HiFi Buffer II is optimized for the amplification of λDNA, cDNA or plasmid DNA.
-
Characteristics
-
- TransTaq® HiFi DNA Polymerase offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.
- Extension rate is about 1-2 kb/min.
- Template-independent "A" can be generated at the 3' end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors.
- Amplification of genomic DNA fragment up to 15 kb. -
Components
- DNA Polymerase, 10X HiFi Buffer I, 10X HiFi Buffer II, 2.5 mM dNTPs, 10X GC Enhancer, 6X DNA Loading Buffer
-
Unit Definition
- One unit of TransTaq® HiFi DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
-
-
-
-
Application Notes
- Complex templates, GC/AT rich templates, Long PCR, High yield PCR
-
Comment
-
TransTaq® HiFi DNA Polymerase has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity, >99% homogeneous measured by SDS-PAGE. Each batch of TransTaq® HiFi DNA Polymerase has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA
-
Restrictions
- For Research Use only
-
-
-
Buffer
-
Storage Buffer: 20 mM Tris-HCl ( pH 8.0), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, 50 % glycerol, stabilizers
10xTransTaq® HiFi Buffer:200 mM Tris-HCl ( pH 9.0), 100 mM KCl, 100 mM (NH4)2SO4, 20 mM MgSO4, 10 % Glycerol, others -
Storage
- -20 °C
-
Storage Comment
- at -20°C for two years
-
Expiry Date
- 24 months
-
-
You are here:
