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E. coli DNA Ligase

Details for Product No. ABIN3188192, Supplier: Log in to see
Application
Ligation (Lig)
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Characteristics E.coli DNA Ligase is an NAD+-dependent enzyme that catalyzes the formation of a phosphodiester bond between cohesive 3'-hydroxyl and 5'-phosphoryl termini of double-stranded DNA (dsDNA). This enzyme is also active on nicked DNA but is not effective for the formation of DNA-RNA or RNA-RNA hybrids.
Components Enzyme supplied with 10X Reaction Buffer
Unit Definition One unit is defined as the amount of E. coli DNA Ligase that is required to give 50% ligation of HindIII-digested λ DNA in a total reaction volume of 20 μl in 30 minutes at 16°C in 1X E.coli DNA Ligase Reaction Buffer (concentration of 5' DNA termini is 0.12 μM [300 μg/ml]).
Comment

  • Ligation of dsDNA with cohesive termini
  • cDNA cloning of products from second strand cDNA synthesis experiments
  • Alternative to T4 DNA Ligase when bluntend ligation is not required
  • For ligation of bluntend fragments use T4 DNA Ligase (abm Cat. No. G467)

Restrictions For Research Use only
Concentration 10 U/μL
Buffer 50 mM Tris-HCl ( pH 7.5), 1 mM EDTA, 1 mM DTT, 200 mM NaCl, and 50 % (v/v) Glycerol.
Storage -20 °C