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Poly(A) Polymerase, Yeast

Details for Product No. ABIN3188236, Supplier: Log in to see
Application
DNA Amplification (DNA Amp)
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Characteristics Poly(A) Polymerase, Yeast catalyses the template independent addition of adenosine residues onto the 3' ends of polyribonucleotides. The use of ATP as a substrate leads to poly(A) tailing whereas substitution of cordycepin-5'-triphosphate (3'-dATP) for ATP results in addition of a single dA residue to the 3'-termini of the RNA. Neither ADP nor dATP can be used as substrates for this enzyme. Poly(A) Polymerase from yeast has been shown to be more effective at oligonucleotide-labeling and poly(A) tailing of long RNA templates than Poly(A) Polymerase from E. coli.
Components Enzyme supplied with 5X Reaction Buffer
Unit Definition One unit is defined as the amount of Poly(A) Polymerase, Yeast that catalyzes the incorporation of 1 nmol of AMP into RNA in 10 minutes at 37°C.
Comment

  • Labelling of RNA with ATP or cordycepin
  • Poly(A) tailing of RNA for cloning or affinity purification
  • Increasing translation of RNA transferred into eukaryotic cells

Restrictions For Research Use only
Concentration 1 U/μL
Buffer 20 mM Tris-HCl ( pH 8.0), 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1 % Triton® X-100 and 50 % (v/v) Glycerol.
Storage -20 °C