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Enzymes

Enzyme describes a broad spectrum of biocatalysts that carry out nearly all of the molecular chemistry necessary for life. Most enzymes are single or multi-subunit proteins that recognize a single substrate, and reduce the activation-energy necessary to convert that substrate into a specific product. Enzymes are particularly useful tools in the molecular biologist’s tool kit. They are widely used in both genomics and proteomics research.

Many different classes of enzymes are used to manipulate genetic sequences. E.g. restriction endonucleases cleave DNA at a specified sequence, polymerases amplify a double stranded nucleotide sequence, and ligases join nucleotide sequences together. Recently, engineered restriction nucleases like Cas9 have gathered significant research interest, as they may be used for targeted excision and replacement of genomic DNA –allowing a simple method for “on-the-fly” editing of a specific gene. We offer a wide variety of enzymes, restriction enzymes, polymerases and more products for different applications. For questions regarding our product portfolio, please contact our customer service via chat, phone, or email.

232 Products
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    Enzyme
Gene
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Species
  • 15
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Host
  • 15
Application
  • 108
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Protein Type
  • 9
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ISO 9001:2008

Application:
DNA Modification
Concentration:
250 U/μL
Storage:
-20 °C
Specificity:
One unit will digest sonicated salmon sperm DNA to acid-soluble oligonucleotides equivalent to a ΔA260 of 1.0 in 30 min at pH 8.0 at 37 ℃, which corresponds approximately to complete digestion of 37 μg DNA. Note that 1 KU=1000 units.
Catalog No. ABIN2180646
$96.80
Will be delivered in 5 Business Days
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Pfu DNA Polymerase for accurate and consistent performance in PCR.

Application:
Polymerase Chain Reaction
Concentration:
2.5 units/μL
Storage:
-20 °C
Specificity:
An enzyme that catalyzes the polymerization of nucleotides
Publications
  • Jin, Zhuo, Guo, Field: "p21-activated Kinase 1 (Pak1)-dependent phosphorylation of Raf-1 regulates its mitochondrial localization, phosphorylation of BAD, and Bcl-2 association." in: The Journal of biological chemistry, Vol. 280, Issue 26, pp. 24698-705, 2005 (Pubmed)
Catalog No. ABIN411757
$441.00
Will be delivered in 2 to 3 Business Days
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Pure recombinant active bacterial TurboNuclease

Application:
DNA Modification
Concentration:
250 U/μL
Storage:
-20 °C
Specificity:
A nonspecific endonuclease
Catalog No. ABIN2506908
$294.00
50000 units
Will be delivered in 2 to 3 Business Days
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FastPfu DNA Polymerase is a fast, high fidelity and high processivity hot start DNA polymerase.

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase
Storage:
-20 °C
Specificity:
Functional absence of double and single stranded endonuclease activity.
Catalog No. ABIN2952634
$187.50
Will be delivered in 9 to 14 Business Days
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FastPfu DNA Polymerase is a fast, high fidelity and high processivity hot start DNA polymerase.

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase (with 2.5 mM dNTPs)
Storage:
-20 °C
Specificity:
Functional absence of double and single stranded endonuclease activity.
Catalog No. ABIN2952635
$187.50
Will be delivered in 9 to 14 Business Days
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Taq DNA Polymerase for PCR and primer extension reactions at elevated temperature.

Application:
Polymerase Chain Reaction
Concentration:
2.5 units/μL
Components:
Taq DNA Polymerase + 10X Reaction Buffer
Storage:
-20 °C
Catalog No. ABIN411754
$441.00
Will be delivered in 2 to 3 Business Days
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Application:
Polymerase Chain Reaction
Storage:
-20 °C
Publications
  • Lee, Rollwagen-Bollens, Bollens, Faber-Hammond: "Environmental influence on cyanobacteria abundance and microcystin toxin production in a shallow temperate lake." in: Ecotoxicology and environmental safety, Vol. 114, Issue , pp. 318-25, 2015 (Pubmed)
  • González, Brito, González: "Identification of glycoproteins secreted by wild-type Botrytis cinerea and by protein O-mannosyltransferase mutants." in: BMC microbiology, Vol. 14, Issue , pp. 254, 2015 (Pubmed)
  • Boyd, Tennant, Melendez, Toema, Galen, Geddes, Levine: "Adaptation of red blood cell lysis represents a fundamental breakthrough that improves the sensitivity of Salmonella detection in blood." in: Journal of applied microbiology, Vol. 118, Issue 5, pp. 1199-209, 2015 (Pubmed)
  • Miersch, Li, Hanna, McLaughlin, Hornsby, Matsuguchi, Paduch, Sääf, Wells, Koide, Kossiakoff, Sidhu: "Scalable high throughput selection from phage-displayed synthetic antibody libraries." in: Journal of visualized experiments : JoVE, Vol. , Issue 95, pp. 51492, 2015 (Pubmed)
  • Thekke-Veetil, Polashock, Marn, Plesko, Schilder, Keller, Martin, Tzanetakis: "Population structure of blueberry mosaic associated virus: Evidence of reassortment in geographically distinct isolates." in: Virus research, Vol. 201, Issue , pp. 79-84, 2015 (Pubmed)
  • Maxfield-Taylor, Mujic, Rao: "First detection of the larval chalkbrood disease pathogen Ascosphaera apis (Ascomycota: Eurotiomycetes: Ascosphaerales) in adult bumble bees." in: PLoS ONE, Vol. 10, Issue 4, pp. e0124868, 2015 (Pubmed)
  • Frías, Brito, González, González: "The phytotoxic activity of the cerato-platanin BcSpl1 resides in a two-peptide motif on the protein surface." in: Molecular plant pathology, Vol. 15, Issue 4, pp. 342-51, 2014 (Pubmed)
  • Acanda, Martínez, Prado, González, Rey: "EMS mutagenesis and qPCR-HRM prescreening for point mutations in an embryogenic cell suspension of grapevine." in: Plant cell reports, Vol. 33, Issue 3, pp. 471-81, 2014 (Pubmed)
  • Zhang, Liu, Liu, Zhang, Cao, Ye, Wang, Zhang, Xiao, Yang, Li, Bai: "Transcriptome profiling of a multiple recurrent muscle-invasive urothelial carcinoma of the bladder by deep sequencing." in: PLoS ONE, Vol. 9, Issue 3, pp. e91466, 2014 (Pubmed)
  • Boyd, Tennant, Saague, Simon, Muhsen, Ramachandran, Cross, Galen, Pasetti, Levine: "Serum bactericidal assays to evaluate typhoidal and nontyphoidal Salmonella vaccines." in: Clinical and vaccine immunology : CVI, Vol. 21, Issue 5, pp. 712-21, 2014 (Pubmed)
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Catalog No. ABIN1536547
$66.00
Will be delivered in 3 to 4 Business Days
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Application:
Polymerase Chain Reaction
Storage:
-20 °C
Publications
  • Laurin-Lemay, Angers, Benrey, Brodeur: "Inconsistent genetic structure among members of a multitrophic system: did bruchid parasitoids (Horismenus spp.) escape the effects of bean domestication?" in: Bulletin of entomological research, Vol. 103, Issue 2, pp. 182-92, 2013 (Pubmed)
Catalog No. ABIN1536548
$163.90
2500 U
Will be delivered in 3 to 4 Business Days
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DNA Polymerase is purified from E. coli expressing a cloned DNA polymerase from Thermus aquaticus.

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase
Storage:
-20 °C
Specificity:
Functional absence of double and single stranded endonuclease activity.
Catalog No. ABIN2952620
$125.00
Will be delivered in 9 to 14 Business Days
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The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa.

Application:
Polymerase Chain Reaction
Storage:
-20 °C
Specificity:
Functional absence of double and single stranded endonuclease activity.
Catalog No. ABIN2952618
$137.50
Will be delivered in 9 to 14 Business Days
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The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa.

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase (with 2.5 mM dNTPs)
Storage:
-20 °C
Specificity:
Functional absence of double and single stranded endonuclease activity.
Catalog No. ABIN2952619
$137.50
Will be delivered in 9 to 14 Business Days
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DNA Polymerase is purified from E. coli expressing a cloned DNA polymerase from Thermus aquaticus.

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase (with 2.5 mM dNTPs)
Storage:
-20 °C
Specificity:
Functional absence of double and single stranded endonuclease activity.
Catalog No. ABIN2952621
$137.50
Will be delivered in 9 to 14 Business Days
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Taq DNA Polymerase is a hot start Taq DNA polymerase containing Taq DNA polymerase and two proprietary DNA binding proteins.

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase
Storage:
-20 °C
Specificity:
Functional absence of double and single stranded endonuclease activity.
Catalog No. ABIN2952628
$162.50
Will be delivered in 9 to 14 Business Days
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Taq DNA Polymerase is a hot start Taq DNA polymerase containing Taq DNA polymerase and two proprietary DNA binding proteins.

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase (with 2.5 mM dNTPs)
Storage:
-20 °C
Specificity:
Functional absence of double and single stranded endonuclease activity.
Catalog No. ABIN2952629
$175.00
Will be delivered in 9 to 14 Business Days
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DNA Polymerase for PAGE is purified from E. coli expressing a cloned DNA polymerase from Thermus aquaticus.

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase
Storage:
-20 °C
Specificity:
Functional absence of double and single stranded endonuclease activity.
Catalog No. ABIN2952622
$175.00
Will be delivered in 9 to 14 Business Days
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DNA Polymerase for PAGE is purified from E. coli expressing a cloned DNA polymerase from Thermus aquaticus.

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase (with 2.5 mM dNTPs)
Storage:
-20 °C
Specificity:
Functional absence of double and single stranded endonuclease activity.
Catalog No. ABIN2952623
$200.00
Will be delivered in 9 to 14 Business Days
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Diamond Taq® polymerase* is a highly thermostable enzyme produced and purified from recombinant Escherichia coli bacterium containing the Thermus aquaticus DNA Polymerase gene.

Application:
Polymerase Chain Reaction, DNA Amplification
Components:
Diamond Taq® is provided at a concentration of 5 U/μl with 10x reaction buffer and MgCl2 solution.
  • 10x Reaction buffer: 750 mM Tris-HCl, 200 mM (NH4)2SO4, 0.1 % (v/v) Tween 20 and stabilizer, pH 8.8 (at 19 °C).
  • MgCl2 solution: 25 mM MgCl2
  • Enzyme storage buffer: 20 mM Tris-HCl, 1 mM DTT, 0.1 mM EDTA, 0.1 M KCl, 0.5 % (v/v) Nonidet P40, 0.5 % (v/v) Tween 20, 50 % (v/v) glycerol and stabilizer pH 8.0 (19 °C)
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of enzyme that incorporates, 10nmoles of dNTPs into acid insoluble form in 30 minutes at 74 °C.
Specificity:
Diamond Taq® polymerase* is a highly thermostable enzyme produced and purified from recombinant Escherichia coli bacterium containing the Thermus aquaticus DNA Polymerase gene. The expressed enzyme catalyzes 5'→3' synthesis of DNA with no detectable 3'→5' proof reading exonuclease activity. This enzyme has the "extendase" activity allowing TA cloning.
Catalog No. ABIN3188324
$103.95
1000 U
Will be delivered in 5 to 6 Business Days
Supplier: Log in to see

Diamond Taq® polymerase* is a highly thermostable enzyme produced and purified from recombinant Escherichia coli bacterium containing the Thermus aquaticus DNA Polymerase gene.

Application:
Polymerase Chain Reaction, DNA Amplification
Components:
Diamond Taq® is provided at a concentration of 5 U/μl with 10x reaction buffer and MgCl2 solution.
  • 10x Reaction buffer: 750 mM Tris-HCl, 200 mM (NH4)2SO4, 0.1 % (v/v) Tween 20 and stabilizer, pH 8.8 (at 19 °C).
  • MgCl2 solution: 25 mM MgCl2
  • Enzyme storage buffer: 20 mM Tris-HCl, 1 mM DTT, 0.1 mM EDTA, 0.1 M KCl, 0.5 % (v/v) Nonidet P40, 0.5 % (v/v) Tween 20, 50 % (v/v) glycerol and stabilizer pH 8.0 (19 °C)
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of enzyme that incorporates, 10nmoles of dNTPs into acid insoluble form in 30 minutes at 74 °C.
Specificity:
Diamond Taq® polymerase* is a highly thermostable enzyme produced and purified from recombinant Escherichia coli bacterium containing the Thermus aquaticus DNA Polymerase gene. The expressed enzyme catalyzes 5'→3' synthesis of DNA with no detectable 3'→5' proof reading exonuclease activity. This enzyme has the "extendase" activity allowing TA cloning.
Catalog No. ABIN3188326
$420.00
5000 U
Will be delivered in 5 to 6 Business Days
Supplier: Log in to see

Diamond Taq® polymerase* is a highly thermostable enzyme produced and purified from recombinant Escherichia coli bacterium containing the Thermus aquaticus DNA Polymerase gene.

Application:
Polymerase Chain Reaction, DNA Amplification
Components:
Diamond Taq® is provided at a concentration of 5 U/μl with 10x reaction buffer and MgCl2 solution.
  • 10x Reaction buffer: 750 mM Tris-HCl, 200 mM (NH4)2SO4, 0.1 % (v/v) Tween 20 and stabilizer, pH 8.8 (at 19 °C).
  • MgCl2 solution: 25 mM MgCl2
  • Enzyme storage buffer: 20 mM Tris-HCl, 1 mM DTT, 0.1 mM EDTA, 0.1 M KCl, 0.5 % (v/v) Nonidet P40, 0.5 % (v/v) Tween 20, 50 % (v/v) glycerol and stabilizer pH 8.0 (19 °C)
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of enzyme that incorporates, 10nmoles of dNTPs into acid insoluble form in 30 minutes at 74 °C.
Specificity:
Diamond Taq® polymerase* is a highly thermostable enzyme produced and purified from recombinant Escherichia coli bacterium containing the Thermus aquaticus DNA Polymerase gene. The expressed enzyme catalyzes 5'→3' synthesis of DNA with no detectable 3'→5' proof reading exonuclease activity. This enzyme has the "extendase" activity allowing TA cloning.
Catalog No. ABIN3188325
$1,984.50
25000 U
Will be delivered in 5 to 6 Business Days
Supplier: Log in to see

Application:
Polymerase Chain Reaction
Storage:
RT
Publications
  • Harada, Takagi, Nakano, Yamaji, Inui: "Protein arginine methyltransferase 10 is required for androgen-dependent proliferation of LNCaP prostate cancer cells." in: Bioscience, biotechnology, and biochemistry, Vol. 79, Issue 9, pp. 1430-7, 2015 (Pubmed)
  • Taboada, Hermida, Pardo, Vera, Piferrer, Viñas, Bouza, Martínez: "Fine mapping and evolution of the major sex determining region in turbot (Scophthalmus maximus)." in: G3 (Bethesda, Md.), Vol. 4, Issue 10, pp. 1871-80, 2014 (Pubmed)
Catalog No. ABIN1536228
$66.00
1000 U
Will be delivered in 3 to 4 Business Days
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T DNA Polymerase is a mixture of DNA Polymerase with proofreading 3’-5’ exonuclease.

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase
Storage:
-20 °C
Specificity:
Functional absence of double and single stranded endonuclease activity.
Catalog No. ABIN2952624
$137.50
Will be delivered in 9 to 14 Business Days
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T DNA Polymerase is a mixture of DNA Polymerase with proofreading 3’-5’ exonuclease.

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase (with 2.5 mM dNTPs)
Storage:
-20 °C
Specificity:
Functional absence of double and single stranded endonuclease activity.
Catalog No. ABIN2952625
$137.50
Will be delivered in 9 to 14 Business Days
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DNA Polymerase High Fidelity (HiFi DNA Polymerase) contains T DNA Polymerase and a proofreading 3’-5’ exonuclease.

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase
Storage:
-20 °C
Specificity:
Functional absence of double and single stranded endonuclease activity.
Catalog No. ABIN2952626
$162.50
Will be delivered in 9 to 14 Business Days
Supplier: Log in to see

DNA Polymerase High Fidelity (HiFi DNA Polymerase) contains T DNA Polymerase and a proofreading 3’-5’ exonuclease.

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase (with 2.5 mM dNTPs)
Storage:
-20 °C
Specificity:
Functional absence of double and single stranded endonuclease activity.
Catalog No. ABIN2952627
$162.50
Will be delivered in 9 to 14 Business Days
Supplier: Log in to see

One binding protein binds to double-stranded DNA template, preventing polymerase activity at room temperature.

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase
Storage:
-20 °C
Specificity:
Functional absence of double and single stranded endonuclease activity.
Catalog No. ABIN2952630
$175.00
Will be delivered in 9 to 14 Business Days
Supplier: Log in to see

One binding protein binds to double-stranded DNA template, preventing polymerase activity at room temperature.

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase (with 2.5 mM dNTPs)
Storage:
-20 °C
Specificity:
Functional absence of double and single stranded endonuclease activity.
Catalog No. ABIN2952631
$175.00
Will be delivered in 9 to 14 Business Days
Supplier: Log in to see

Application:
Restriction Digest
Components:
50 Units PvuRts1 I restriction enzyme
10X PvuRts1 I Reaction Buffer
1 M DTT
Storage:
-20 °C/-80 °C
Specificity:
PvuRts1 I restriction enzyme recognizes the sequence hmCN11-12/N9-10G.
Catalog No. ABIN2669403
$274.17
50 units
Will be delivered in 1 to 2 Business Days
Supplier: Log in to see

T4 DNA Ligase (5 u/ul)

Application:
Ligation
Concentration:
5 U/μL
Storage:
-80 °C
Specificity:
An enzyme which anneals DNA strands together
Catalog No. ABIN411759
$126.00
250 U
Will be delivered in 2 to 3 Business Days
Supplier: Log in to see

EasyPfu DNA Polymerase possesses a proofreading 3'-5' exonuclease activity.

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase
Storage:
-20 °C
Specificity:
Functional absence of double and single stranded endonuclease activity.
Catalog No. ABIN2952632
$137.50
Will be delivered in 9 to 14 Business Days
Supplier: Log in to see

EasyPfu DNA Polymerase possesses a proofreading 3'-5' exonuclease activity.

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase (with 2.5 mM dNTPs)
Storage:
-20 °C
Specificity:
Functional absence of double and single stranded endonuclease activity.
Catalog No. ABIN2952633
$137.50
Will be delivered in 9 to 14 Business Days