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Exonuclease I, E. coli

Details for Product No. ABIN3188195, Supplier: Log in to see
Application
DNA Modification (DNA Mod)
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Characteristics Exonuclease I, E.coli catalyzes the removal of nucleotides from single-stranded DNA in the 3'→5' direction, releasing deoxyribonucleoside 5'-monophosphates and leaving 5'-terminal dinucleotides intact. Hydrolysis cannot proceed if the 3'-terminus is phosphorylated.
Components Enzyme supplied with 10X Reaction Buffer
Unit Definition One unit is defined as the amount of Exonuclease I, E. coli that is required to catalyze the release of 10 nmol of acid soluble nucleotides from heat-denatured DNA in 30 minutes at 37°C in 1X Exonuclease I Reaction Buffer.
Comment

  • Removal of singlestranded primer oligonucleotides from:
  • PCR mixtures for applications involving sequencing or labelling
  • from nucleic acid mixtures
  • Assaying for the presence of singlestranded DNA with a 3'hydroxyl terminus

Restrictions For Research Use only
Concentration 20 U/μL
Buffer 20 mM HEPES ( pH 7.1), 150 mM NaCl, 1 mM DTT, 1 mM EDTA, and 50 % (v/v) Glycerol.
Storage -20 °C