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T4 DNA Ligase

Details for Product No. ABIN3188209, Supplier: Log in to see
Application
Ligation (Lig)
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Characteristics T4 DNA Ligase catalyzes the formation of a phosphodiester bond between the proximal 3'- hydroxyl and 5'- phosphate ends of adjacent, nicked nucleotides of dsDNA, dsRNA or DNA-RNA hybrids. Known to proceed via an adenylated intermediate, this endergonic reaction can be utilized to join both cohesive-ended and blunt-ended termini.
Components Enzyme supplied with 5X Reaction Buffer
Comment

  • Cloning of restriction fragments
  • Attaching doublestranded oligonucleotide linkers or adaptors to DNA
  • Sitedirected mutagenesis
  • Nick repair of duplex DNA, RNA or DNARNA hybrids
  • Selfcircularization of linear DNA

Restrictions For Research Use only
Concentration 1 U/μL
Storage -20 °C