TransTaq® DNA Polymerase High Fidelity(HiFi)

Details for Product No. ABIN5519576, Supplier: Log in to see
Application
Polymerase Chain Reaction (PCR)
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Purpose TransTaq® DNA Polymerase High Fidelity (TransTaq® HiFi DNA Polymerase) contains TransTaq®-T DNA Polymerase and a proofreading 3'-5' exonuclease.
Brand TransTaq®
Specificity TransTaq® HiFi DNA Polymerase provides higher specificity and higher amplification efficiency than TransTaq®-T DNA Polymerase. Two different buffers are provided in the kit. TransTaq® HiFi Buffer I is optimized for the amplification of genomic DNA and TransTaq® HiFi Buffer II is optimized for the amplification of λDNA, cDNA or plasmid DNA.
Characteristics - TransTaq® HiFi DNA Polymerase offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.
- Extension rate is about 1-2 kb/min.
- Template-independent "A" can be generated at the 3' end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors.
- Amplification of genomic DNA fragment up to 15 kb.
Components DNA Polymerase, 10X HiFi Buffer I, 10X HiFi Buffer II, 10X GC Enhancer, 6X DNA Loading Buffer
Unit Definition One unit of TransTaq® HiFi DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
Application Notes Complex templates, GC/AT rich templates, Long PCR, High yield PCR
Comment

TransTaq® HiFi DNA Polymerase has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity, >99% homogeneous measured by SDS-PAGE. Each batch of TransTaq® HiFi DNA Polymerase has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA

Restrictions For Research Use only
Buffer Storage Buffer: 20 mM Tris-HCl ( pH 8.0), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, 50 % glycerol, stabilizers
10xTransTaq® HiFi Buffer:200 mM Tris-HCl ( pH 9.0), 100 mM KCl, 100 mM (NH4)2SO4, 20 mM MgSO4, 10 % Glycerol, others
Storage -20 °C
Storage Comment at -20°C for two years
Expiry Date 24 months
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