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RecA Protein

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Characteristics RecA from E. coli is a DNA-binding protein that is involved in homologous recombination in an ATP-dependent process. RecA binds to single-stranded DNA forming a nucleoprotein complex and promotes the strand exchange of single-strand DNA fragments with homologous duplex DNA. RecA also plays a role in post-replicative DNA repair mechanisms and in DNA repair and UV-induced mutagenesis. RecA Protein is commonly used to study the molecular mechanisms involved in homologous recombination.
Components Protein supplied with 10X Reaction Buffer.
Comment

  • displacement loop mutagenesis
  • targeted DNA cleavage
  • visualization of DNA with electron microscopy
  • library screening with RecA coated probes

Restrictions For Research Use only
Concentration 2 mg/mL
Buffer 10 mM Tris-HCl ( pH 7.5), 0.1 mM EDTA, 1 mM DTT, and 50 % (v/v) Glycerol.
Storage -20 °C